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نتائج ل
"Wright, Vonda"
صنف حسب:
Synergistic enhancement of bone formation and healing by stem cell-expressed VEGF and bone morphogenetic protein-4
We investigated the interaction between angiogenic and osteogenic factors in bone formation and bone healing with ex vivo gene therapy using muscle-derived stem cells genetically engineered to express human bone morphogenetic protein-4 (BMP4), VEGF, or VEGF-specific antagonist (soluble Flt1). Our results show that although VEGF alone did not improve bone regeneration, it acted synergistically with BMP4 to increase recruitment of mesenchymal stem cells, to enhance cell survival, and to augment cartilage formation in the early stages of endochondral bone formation. These early effects, coupled with accelerated cartilage resorption, eventually led to a significant enhancement of bone formation and bone healing. The beneficial effect of VEGF on bone healing elicited by BMP4 depends critically on the ratio of VEGF to BMP4, with an improper ratio leading to detrimental effects on bone healing. Finally, we show that soluble Flt1 inhibits bone formation elicited by BMP4. Thus, VEGF plays an important role in bone formation elicited by BMP4, and it can significantly enhance BMP4-elicited bone formation and regeneration through multiple mechanisms. This study has important implications for the formulation of new strategies to improve bone healing through increasing mesenchymal stem cell recruitment and survival, in combination with muscle-derived stem cell-based gene therapy.
Journal Article
Fitness after 40 : your strong body at 40, 50, 60, and beyond
\"Includes a 6-week total-body workout program.\"
Book
BMP4-Expressing Muscle-Derived Stem Cells Differentiate into Osteogenic Lineage and Improve Bone Healing in Immunocompetent Mice
Recent advances in molecular biology have led the way for novel approaches to improve bone healing. The ideal growth factor, vector, and delivery systems for producing bone in an immune competent animal model, however, have yet to be identified. Using a retrovirus encoding BMP4 and recently isolated muscle-derived stem cells (MDSCs), we demonstrated the following: MDSCs undergo osteogenic differentiation in response to BMP4 in a dose-dependent manner; retrovirus encoding BMP4 can efficiently transduce MDSCs, both enhancing osteogenic differentiation and inhibiting myogenic differentiation; transduced MDSCs can produce high levels of functional BMP4 as they differentiate toward an osteogenic lineage; allogeneic transduced MDSCs can induce robust de novo bone formation in immunocompetent mice despite the presence of an immune reaction, demonstrating the ability of this retroviral-BMP4-muscle construct to provide sufficient stimuli for osteoinduction in vivo; MDSCs appear to deliver BMP4, respond to the human BMP4 in an autocrine manner, and actively participate in bone formation, thus serving both osteoinductive and osteoproductive roles; and the BMP4-expressing MDSCs can induce bone formation and improve bone healing in a critical-sized skull defect in immunocompetent mice. Therefore, we believe that technology based on the MDSCs and vector system has great potential for promoting bone healing in a variety of musculoskeletal conditions.
Journal Article
Metadata for Graphic Novels and Comic Books: Comic Book Markup Language and Advanced Comic Book Format
Graphic novels and comics can be difficult to catalog due to the graphic and textual nature of the medium. The most popular and used formats for comics and graphic novels would not met the standard for most institutions, but since there are a number of digital files already available in the Comic book RAR (CBR) and Comic Book Zip(CBZ) reader file format with little to no metadata attached those files should be cataloged using either a known xml or other schema and then have a crosswalk applied to them to adapt them to the more controlled, and more complex, Comic Book Markup Language (CBML) that should be used by information institutions. CBML is based in Text Encoding Initiative (TEI), while the standard for comic books as it can be added to XML such as Dublin Core, and is extremely involved and far more complicated than the average institution will need. The project purposed is a set of records created using specific standard for metadata cataloging of comics and graphic novels, CBR and CBZ reader files, in Dublin Core (DC) with a detailed crosswalk to the more Advanced Comic Book Format with the CBML in the appropriate sections.
Journal Article
Synergistic enhancement of bone formation and healing by stem cell--expressed VEGF and bone morphogenetic protein-4
We investigated the interaction between angiogenic and osteogenic factors in bone formation and bone healing with ex vivo gene therapy using muscle-derived stem cells genetically engineered to express human bone morphogenetic protein-4 (BMP4), VEGF, or VEGF-specific antagonist (soluble Flt1). Our results show that although VEGF alone did not improve bone regeneration, it acted synergistically with BMP4 to increase recruitment of mesenchymal stem cells, to enhance cell survival, and to augment cartilage formation in the early stages of endochondral bone formation. These early effects, coupled with accelerated cartilage resorption, eventually led to a significant enhancement of bone formation and bone healing. The beneficial effect of VEGF on bone healing elicited by BMP4 depends critically on the ratio of VEGF to BMP4, with an improper ratio leading to detrimental effects on bone healing. Finally, we show that soluble Flt1 inhibits bone formation elicited by BMP4. Thus, VEGF plays an important role in bone formation elicited by BMP4, and it can significantly enhance BMP4-elicited bone formation and regeneration through multiple mechanisms. This study has important implications for the formulation of new strategies to improve bone healing through increasing mesenchymal stem cell recruitment and survival, in combination with muscle-derived stem cell-based gene therapy.
Journal Article
Holiday snuggling with books
READING CORNER The holiday season is in full swing and it's a perfect time to snuggle up with loved ones and read a few holiday books. Whether the celebration is Christmas, Hanukkah, Kwanzaa or something in between, a perfect book is ready to share with the family. To teach the seven principles of Kwanzaa, seven quarrelsome brothers are set an impossible task of turning thread into gold if they want to receive any inheritance from their father.
Newspaper Article
The self-perception of African-American pre-adolescents as it relates to their career aspirations
The purpose of this study was to determine how African-American pre-adolescent boys and girls perceive themselves across six different domains, and to determine if their perceptions are related to their career aspirations. Thirty-five African-American fifth and sixth grade students were administered the Self-Perception Scale for Children. After completing the scale, the students were instructed to write down three careers that they wanted to pursue when they grew up, in order of preference. The students had the option of choosing from a list of careers that was provided to them, or of making a career choice on their own. The results indicated a statistical difference between boys and girls on the scholastic and athletic domains only, with boys perceiving themselves to be more proficient than girls. The results also showed that boys chose professional athlete as a first career choice 34.3% of the time, and 88.6% of all the first career choices were clustered in five highly visible professions. Suggestions are made to incorporate self-esteem and career counseling into the regular classroom curriculum.
Dissertation